A fascinating study revealing that TgCDPK1 is stabilized and activated via an allosteric mechanism involving its regulatory CAD domain, challenging classical models of calcium-dependent kinase activation.
A landmark structural study revealing how apicomplexan CDPKs (TgCDPK1/3, CpCDPK) undergo dramatic conformational changes upon calcium binding to shift from an autoinhibited to an active state.
A landmark structural study revealing how apicomplexan CDPKs (TgCDPK1/3, CpCDPK) undergo dramatic conformational changes upon calcium binding to shift from an autoinhibited to an active state.
Notes on a 2001 Journal of Molecular Biology paper revealing the crystal structure of Ca2+/CaM bound to a CaMKK-derived peptide, highlighting how calmodulin achieves conformational flexibility to accommodate diverse targets — offering a mechanistic basis for how CaM relieves autoinhibition of kinases like CaMKK and mediates calcium signal transduction.
Notes on a 2001 Journal of Molecular Biology paper revealing the crystal structure of Ca2+/CaM bound to a CaMKK-derived peptide, highlighting how calmodulin achieves conformational flexibility to accommodate diverse targets — offering a mechanistic basis for how CaM relieves autoinhibition of kinases like CaMKK and mediates calcium signal transduction.
Note on the detailed structural study of CDPK activation, focusing on apicomplexan CAD conformations (CpCDPK, PfCDPK3), offering new insight into a potentially universal activation mechanism for CDPKs.
Note on the detailed structural study of CDPK activation, focusing on apicomplexan CAD conformations (CpCDPK, PfCDPK3), offering new insight into a potentially universal activation mechanism for CDPKs.
A 1975 Science paper pioneering the computational prediction of EF-hand calcium-binding motifs in unexpected proteins, revealing evolutionary insights into calcium sensor domains and highlighting structural conservation even in non-calcium-binding proteins.
A 1975 Science paper pioneering the computational prediction of EF-hand calcium-binding motifs in unexpected proteins, revealing evolutionary insights into calcium sensor domains and highlighting structural conservation even in non-calcium-binding proteins.
A 1991 Science paper reporting the discovery of a soybean CDPK as a novel class of calcium-dependent kinases, providing structural and evolutionary insights that shaped the understanding of CDPKs as CaM-like autonomous calcium sensors.
A 1991 Science paper reporting the discovery of a soybean CDPK as a novel class of calcium-dependent kinases, providing structural and evolutionary insights that shaped the understanding of CDPKs as CaM-like autonomous calcium sensors.
Notes on a 2004 JBC paper exploring differential roles of N- and C-lobes in the CaM-like domain (CaM-LD) of CDPK, uncovering distinct Ca2+ affinities and their role in regulating kinase activation through interactions with the junction domain.
Notes on a 2004 JBC paper exploring differential roles of N- and C-lobes in the CaM-like domain (CaM-LD) of CDPK, uncovering distinct Ca2+ affinities and their role in regulating kinase activation through interactions with the junction domain.
