Notes on 1998 Cell review discussing kinase phosphoryl transfer mechanisms, conformational dynamics, cofactor roles, and binding motifs. Highlights the debate between associative (SN2) and dissociative (SN1) pathways, with structural evidence for both models.
A landmark review on the ordered, site-specific phosphorylation events governing the maturation, activation, and regulation of ABC kinases — using PKC as a paradigm — with profound insights into kinase life cycles, structural stabilization, and multi-step control mechanisms.
A landmark review on the ordered, site-specific phosphorylation events governing the maturation, activation, and regulation of ABC kinases — using PKC as a paradigm — with profound insights into kinase life cycles, structural stabilization, and multi-step control mechanisms.
Notes on a 2011 ACS Biochemistry study showing that CaMKKβ, unlike CaMKKα, achieves high autonomous activity via intramolecular autophosphorylation at Thr482, partially releasing itself from autoinhibition and making it Ca2+/CaM-independent — revealing a unique kinase regulation mechanism within the CaMKK family.
Notes on a 2011 ACS Biochemistry study showing that CaMKKβ, unlike CaMKKα, achieves high autonomous activity via intramolecular autophosphorylation at Thr482, partially releasing itself from autoinhibition and making it Ca2+/CaM-independent — revealing a unique kinase regulation mechanism within the CaMKK family.
Notes on a 2017 Scientific Reports study proposing the Calmodulin-Fused Kinase (CFK) family as the principal system for converting calcium signals to protein phosphorylation responses in plants, based on deep phylogenetic analysis tracing their evolutionary origins and functional diversification.
Notes on a 2017 Scientific Reports study proposing the Calmodulin-Fused Kinase (CFK) family as the principal system for converting calcium signals to protein phosphorylation responses in plants, based on deep phylogenetic analysis tracing their evolutionary origins and functional diversification.
Notes on a 1992 Science paper introducing the concept of ‘calmodulin trapping’ by CaMKII via Thr286 autophosphorylation, establishing a mechanism for potentiating and decoding transient calcium signals through enhanced CaM binding.
Notes on a 1992 Science paper introducing the concept of ‘calmodulin trapping’ by CaMKII via Thr286 autophosphorylation, establishing a mechanism for potentiating and decoding transient calcium signals through enhanced CaM binding.
Note on the seminal 1994 paper unveiling calmodulin’s dual role in CaMKII autophosphorylation and proposing a model for calcium frequency decoding in neurons. Includes reflections on CDPK parallels and a comparative framework for calcium signaling kinases.
Note on the seminal 1994 paper unveiling calmodulin’s dual role in CaMKII autophosphorylation and proposing a model for calcium frequency decoding in neurons. Includes reflections on CDPK parallels and a comparative framework for calcium signaling kinases.
Notes on a 2010 JBC paper investigating in vivo phosphorylation of tobacco CDPKs (NtCDPK2 and NtCDPK3) in response to stress, revealing distinct intra- and inter-molecular phosphorylation events, N-terminal-specific regulation, and the critical role of membrane localization.
Notes on a 2010 JBC paper investigating in vivo phosphorylation of tobacco CDPKs (NtCDPK2 and NtCDPK3) in response to stress, revealing distinct intra- and inter-molecular phosphorylation events, N-terminal-specific regulation, and the critical role of membrane localization.
Notes on a 1999 ASPS paper analyzing autophosphorylation-dependent activation of a calcium-dependent protein kinase (GnCDPK) from groundnut and its role in substrate phosphorylation.
Notes on a 1999 ASPS paper analyzing autophosphorylation-dependent activation of a calcium-dependent protein kinase (GnCDPK) from groundnut and its role in substrate phosphorylation.
