A 2006 JMB study presenting the crystal structure of Arabidopsis CDPK’s regulatory domain, revealing a domain-swapped dimer and an unexpected mechanism of calmodulin-like domain (CaM-LD) recognition, challenging classical calmodulin-target models.
A 2006 JMB study presenting the crystal structure of Arabidopsis CDPK’s regulatory domain, revealing a domain-swapped dimer and an unexpected mechanism of calmodulin-like domain (CaM-LD) recognition, challenging classical calmodulin-target models.
Notes on a 2004 JBC paper exploring differential roles of N- and C-lobes in the CaM-like domain (CaM-LD) of CDPK, uncovering distinct Ca2+ affinities and their role in regulating kinase activation through interactions with the junction domain.
Notes on a 2004 JBC paper exploring differential roles of N- and C-lobes in the CaM-like domain (CaM-LD) of CDPK, uncovering distinct Ca2+ affinities and their role in regulating kinase activation through interactions with the junction domain.
Notes on a 2004 JBC paper uncovering the high-resolution structure of the Ca2+-regulatory domain of soybean CDPK-alpha, revealing novel lobe interactions and a pivot-based mechanism, distinct from calmodulin-based models.
Notes on a 2004 JBC paper uncovering the high-resolution structure of the Ca2+-regulatory domain of soybean CDPK-alpha, revealing novel lobe interactions and a pivot-based mechanism, distinct from calmodulin-based models.
Notes on a 1996 Biochemistry paper revealing that activation of CDPK involves intramolecular binding between its calmodulin-like domain (CaM-LD) and junction/autoinhibitor, offering deep insights into Ca2+-regulated protein kinase activation.
Notes on a 1996 Biochemistry paper revealing that activation of CDPK involves intramolecular binding between its calmodulin-like domain (CaM-LD) and junction/autoinhibitor, offering deep insights into Ca2+-regulated protein kinase activation.
Notes on a 2000 Plant Journal paper identifying dual lipid modifications — myristoylation and palmitoylation — as essential for membrane targeting of rice CDPK (OsCDPK2), marking the first report of such dual acylation in plant CDPKs.
Notes on a 2000 Plant Journal paper identifying dual lipid modifications — myristoylation and palmitoylation — as essential for membrane targeting of rice CDPK (OsCDPK2), marking the first report of such dual acylation in plant CDPKs.
Notes on a 2010 JBC paper investigating in vivo phosphorylation of tobacco CDPKs (NtCDPK2 and NtCDPK3) in response to stress, revealing distinct intra- and inter-molecular phosphorylation events, N-terminal-specific regulation, and the critical role of membrane localization.
Notes on a 2010 JBC paper investigating in vivo phosphorylation of tobacco CDPKs (NtCDPK2 and NtCDPK3) in response to stress, revealing distinct intra- and inter-molecular phosphorylation events, N-terminal-specific regulation, and the critical role of membrane localization.
Notes on the foundational 1991 Science paper introducing a sequence-based method to predict coiled-coil domains. This paper outlines a statistical framework using heptad repeat patterns, Gaussian distributions, and sliding windows, uncovering over 200 proteins in GenBank with potential coiled-coil motifs.
Notes on the foundational 1991 Science paper introducing a sequence-based method to predict coiled-coil domains. This paper outlines a statistical framework using heptad repeat patterns, Gaussian distributions, and sliding windows, uncovering over 200 proteins in GenBank with potential coiled-coil motifs.
Notes on the 2021 Nature Communications paper revealing a calcium-dependent APPL1-dynein mechanism that coordinates collective EGFR trafficking to the perinuclear region following stimulation.
