Notes on a 1999 ASPS paper analyzing autophosphorylation-dependent activation of a calcium-dependent protein kinase (GnCDPK) from groundnut and its role in substrate phosphorylation.
Comprehensive overview of Histidine Protein Kinases (HPKs), their role in two-component signaling systems across bacteria and non-animal eukaryotes, their domain structure, mechanism, and evolutionary significance.
Notes on the foundational 1991 Science paper introducing a sequence-based method to predict coiled-coil domains. This paper outlines a statistical framework using heptad repeat patterns, Gaussian distributions, and sliding windows, uncovering over 200 proteins in GenBank with potential coiled-coil motifs.
Comprehensive review of kalirin (KALRN) as a regulator of synaptic plasticity, its molecular domains and isoforms, and its emerging link to neurodevelopmental and neuropsychiatric disorders.
Notes on the 2021 Nature Communications paper revealing a calcium-dependent APPL1-dynein mechanism that coordinates collective EGFR trafficking to the perinuclear region following stimulation.
Notes on the 2023 eLife review analyzing protein kinase activation loop autophosphorylation. Discusses cis vs. trans mechanisms, dimerization, regulation, and the biochemical, structural, and kinetic evidence for these processes, while addressing the enigmatic nature of autophosphorylation.
Comprehensive notes on the 2001 Chemical Reviews paper exploring the catalytic and kinetic mechanisms of protein kinases, with PKA as a model system. Covers substrate recognition, metal ion roles, phosphoryl transfer mechanisms, activation loop function, and rate-determining steps.
